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A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
List of contents
The Bacteria.- Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources.- Detection and Purification of Bacillus cereus Enterotoxins.- Campylobacter.- Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces.- Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex.- PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis.- Plesiomonas shigelloides.- Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies.- Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus.- Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples.- HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes.- The Viruses.- Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR.- Detection of Enteroviruses.- Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification.- The Parasites.- Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis.- Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish.- Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.).- Parallel Studies to the Analysis of Food-Borne Pathogens.- Approaches to Developing Quantitative Risk Assessment Models.- A Review of Surveillance Networks of Food-Borne Diseases.
Summary
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
