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This new edition of Natural Products Isolation has been not only significantly updated to reflect recent advances in the field, but has also been expanded with three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. The book includes powerful cutting-edge techniques and simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Building on the high reputation of the first edition of Natural Products Isolation, the authors have in this second edition not only significantly updated all the techniques to reflect recent advances in the field, but also added three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. These readily reproducible methods make it possible for even the novice to extract, prepare, and isolate natural products from given source materials. The book includes not only powerful cutting-edge techniques, but also simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Natural Products Isolation, Second Edition, offers the aspiring natural products researcher all the information needed to attain mastery of essential methods and the more experienced investigator a useful reference guide to all the available techniques.
List of contents
Natural Product Isolation: An Overview
Satyajit D. Sarker, Zahid Latif, and Alexander I. Gray
Initial and Bulk Extraction
Véronique Seidel
Supercritical Fluid Extraction
Lutfun Nahar and Satyajit D. Sarker
An Introduction to Planar Chromatography
Simon Gibbons
Isolation of Natural Products by Low-Pressure Column Chromatography
Raymond G. Reid and Satyajit D. Sarker
Isolation by Ion-Exchange Methods
David G. Durham
Separation by High-Speed Countercurrent Chromatography
James B. McAlpine and Patrick Morris
Isolation by Preparative High-Performance Liquid Chromatography
Zahid Latif
Hyphenated Techniques
Satyajit D. Sarker and Lutfun Nahar
Purification by Solvent Extraction Using Partition Coefficient
Hideaki Otsuka
Crystallization in Final Stages of Purification
Alastair J. Florence, Norman Shankland, and Andrea Johnston
Dereplication and Partial Identification of Compounds
Laurence Dinan
Extraction of Plant Secondary Metabolites
William P. Jones and A. Douglas Kinghorn
Isolation of Marine Natural Products
Wael E. Houssen and Marcel Jaspars
Isolation of Microbial Natural Products
Russell A. Barrow
Purification of Water-Soluble Natural Products
Yuzuru Shimizu and Bo Li
Scale-Up of Natural Product Isolation
Steven M. Martin, David A. Kau, and Stephen K. Wrigley
Follow-Up of Natural Product Isolation
Richard J. P. Cannell
Index
Report
Aus den Rezensionen zur 2. Auflage: "... Aufgeteilt in 18 Kapitel, stellt das Buch einen praktischen step-by-step Leitfaden für das Labor dar, gleichermaßen für den erfahrenen Forscher als auch für Neulinge. Jedes Kapitel vermittelt auch fundiertes Hintergrundwissen zu der jeweiligen Methodik. ... Die Kapitel beinhalten einen Fundus von Praktiker-Tipps zur Vermeidung von bekannten und unbekannten experimentellen Schwierigkeiten. Zahlreiche Referenzen helfen dem interessierten Forscher weiter. Das Werk ist allen an der Materie Interessierten wärmstens zu empfehlen." (CS, in: CLB - Chemie in Labor und Biotechnik, 2006, Vol. 57, Issue 1, S. 36)
