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This Text Book acquires knowledge on Tissue culture techniques deals with the Preparation of tissue culture medium from commercial powder, the Role of serum in cell culture, Isolation of lymphocytes by density gradient centrifugation, Primary cell culture; Preparation of single cell suspension from spleen, Trypsinization of monolayer and subculturing, Cell counting and viability determination, MTT Assay for cell viability and growth, Preparation of metaphase chromosomes from primary lymphocyte culture, Cryopreservation and thawing, Isolation of RNA, Isolation of plasmid DNA and characterization, Isolation of DNA from tissues by phenol, Separation of the erythrocyte membrane, and Mitosis in root tips with technical aspects in detailed explanation.
About the author
Dr. R. Subashini, M.Sc., M.Phil., Ph.D., FSAB, Professora Assistente no Departamento de Bioquímica, D.G.Govt. Arts College for women da Universidade de Annamalai. Ganhou vários prémios, incluindo o BEST TEACHER AWARD, publicou mais de 25 livros. Foi membro do Conselho de Estudos, proferiu palestras em várias conferências nacionais e internacionais.
