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This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.
List of contents
Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens.- Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions.- Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish.- Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells.- High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model.- Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.- Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol.- ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells.- Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids.- Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs).- Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.- Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo.- Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells.- Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.- A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells.- Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation.- A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation.- Osteogenic Differentiation from Mouse Embryonic Stem Cells.- Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation.- Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions.- Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.- Accessing the Human Pluripotent Stem Cell Translatome byPolysome Profiling.- Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors.- Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS.
