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This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
List of contents
Monitoring Mitochondrial Respiration in Mouse Cerebellar Granule Neurons.- Examining Mitochondrial Morphology in Mouse Brains.- Investigating Microglial Ultrastructural Alterations and Intimate Relationships with Neuronal Stress, Dystrophy, and Degeneration in Mouse Models of Alzheimer's Disease.- Performing Single Cell Clonal Analysis in the Mouse Brain using Mosaic Analysis with Double Markers (MADM).- Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Murine Cortical Stroke with Persistent Sensorimotor Deficit.- Modeling Axonal Degeneration Using Motor Nerve Organoids.- Monitoring Autophagy in Neural Stem and Progenitor Cells.- Direct FACS Isolation of Neural Stem/Progenitor Lineages from the Adult Brain.- Analysis of mRNA Dynamics using RNA Sequencing Data.- Assessment of Dopaminergic Neurodegeneration in Mice.- Super-Resolution Imaging of Cytoskeletal Networks in Fixed Brain Tissue.- Spinal Cord Injury in the Mouse using the Infinite Horizons Spinal Cord Impactor.- Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples.- Three-Dimensional Atlas of the Human Amygdala Subnuclei Constructed using Immunohistochemical and Ultra-High Field Magnetic Resonance Imaging Data.- Using Single-Molecule Fluorescence Microscopy to Uncover Neuronal Vulnerability to Protein Damage.- Autopsy Human Brain Dissection Protocol for Common Age-Related Neurodegenerative Disorders.- In Vitro Brain Organoids and Computational Models to Study Cell Death in Brain Diseases.- Isolation of Mouse Embryonic Neural Stem Cells and Characterization of Neural Stem Markers by Flow Cytometry.- Assessment of Neuronal Cell Death in Caenorhabditis elegans.- Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells.- Isolation of Adult Mouse Neural Stem Cells and Assessment of Self-Renewal by ELDA.
