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This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols.
Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
List of contents
Historical DNA manipulation Overview.- Tools for efficient genome editing; ZFN, TALEN, and CRISPR.- Efficient generation of stable cell lines with inducible neuronal transgene expression using the piggyBac transposon system.- Modifying Bacterial Artificial Chromosomes for Extended Genome Modification.- Site specific editing of the genome in Pluripotent cells.- Targeting the AAVS1 site with transgene cassette by CRISPR/Cas9 with an inducible transgene cassette for the to neuronal differentiation of human pluripotent stem cells.- Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour.- CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos.- CRISPR/Cas mutagenesis to generate novel traits in Bactrocera tryoni for Sterile Insect Technique.- CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly.- Generation of gene drive mice for invasive pest population suppression.- Generation of a human deafness sheep model using the CRISPR/Cas system.- Targeted Gene Editing in Porcine Germ Cells.- Generating a Heat Tolerance Mouse Model.- Generation of pigs that produce single sex progeny.- Generation of Double-muscled Sheep and Goats by CRISPR/Cas9-mediated Knockout of the Myostatin Gene.- Regulatory and policy considerations around applications of genome editing in agriculture.
