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This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
List of contents
Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes.- Multi-Dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.- Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades.- Structural Characterization of Multienzyme Assemblies: An Overview.- Assessing Protein Interactions for Clustering of Mitochondrial Urea Cycle Enzymes.- DNA Nano-Scaffolds for Multienzyme Systems Assembly.- Strategies for Multienzyme Assemblies.- Random and Positional Immobilization of Multi-Enzyme Systems.- Compartmentalized Immobilization of Multi-Enzyme Systems.- 3D Printed Polylactic Acid Well-Plate for Multi-Enzyme Immobilization.- Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing The Cucurbit[8]Uril Macrocyclic Host.- Lectin-Mediated Coimmobilization of Cascade Glycoenzymes.- Self-Assembled Multienzyme Nanostructures for Biocatalysis In Cellulo.- Nanoarmored Multi-Enzyme Cascade Catalysis.- Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds.- A Four-Enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-Step Cascade Reactions.- A Bi-Enzymatic Immobilized Nanobiocatalyst for The Biotransformation of Oleuropein to Hydroxytyrosol.- A Simple Recombinant E. coli Cell Lysate-Based Biocatalyst for ATP-Dependent Multi-Step Reactions.- CO2 to Methanol: A Highly Efficient Enzyme Cascade.- Multienzyme Catalysis in Phase-Separated Protein Condensates.- Robust and Continuous Lipase-Catalyzed Reactions in Deep Eutectic Solvents: Low Viscosity and Double CLEA-Lentikats Immobilization (CLEA-LK-Lipases).- Enzymatic Photometric Assays for the Selective Detection of Halides.
