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This detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence.
Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
List of contents
Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection.- A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria.- Predicting Type III Effector Proteins Using the Effectidor Web-Server.- Assay for Type III Secretion in Escherichia coli.- Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica.- Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay.- Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility.- Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes.- Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages.- Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments.- Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections.- Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing.- Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers.- Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce.- Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity.- Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis.- Mouse Model to Study Salmonella-Induced Colitis.- Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model.- In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella.- Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks.
