Hepatitis B and D Protocols - Volume 1: Detection, Genotypes, and Characterization

For volume 1:
A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals.
For both volumes:
A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients. Despite the availability of an effective vaccine, there are still 400 million people worldwide who are chronically infected with the hepatitis B and D viruses (HBV and HDV), and more work still needs to be done in all of the key areas of managing the disease. In Hepatitis B and D Protocols, leading investigators and clinicians have joined forces to create a broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals.
Taken together, Hepatitis B and D Protocols, Volume 1: Detection, Genotypes, and Characterization and Volume 2: Immunology, Model Systems, and Clinical Studies offer both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, as well as an essential resource for finding new therapies to treat chronically infected patients.

List of contents

Part I. Detection of Viral Markers
Overview of Commercial HBV Assay Systems
Stefan Zeuzem
Detection of HBV DNA in Serum Using a PCR-Based Assay
Hau Tim Chung
Detection of HBV DNA by Oligonucleotide Probing
Hsiang Ju Lin
Detection of HBV RNA in Serum of Patients
Wei Zhang, Hans Jörg Hacker, Maria Mildenberger, Qin Su, and Claus H. Schröder
Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR
Scott Bowden, Kathy Jackson, Margaret Littlejohn, and Stephen Locarnini
In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections
Vicky C. H. Lai and Johnson Y. N. Lau
Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis
Wolfram H. Gerlich, Ulrike Wend, and Dieter Glebe
In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approach
Jane W. S. Fang and Johnson Y. N. Lau
Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting
Jie Liu and Mark A. Feitelson
Detection of Serum HDV-RNA by RT-PCR
Antonina Smedile, Maria Grazia Niro, and Mario Rizzetto
Nonradioisotopic In Situ Hybridization for HDV RNA
Francesco Negro
Detection and Characterization of Small and Large HDV Antigens
Thomas B. Macnaughton and Michael M. C. Lai
Immunohistochemical Detection of Hepatitis Delta Antigen
Karim Abid and Francesco Negro
Part II. Viral Genotypes and Variants
HBV Vaccine-Escape Variants
Ashraf A. Basuni and William F. Carman
HBV Genotyping and Analysis for Unique Mutations
Anna Ayres, Stephan Locarnini, and Angeline Bartholomeusz
A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus
Chiaho Shih and Ta-Tung Thomas Yuan
Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion
Chiaho Shih and Pei-Ching Tai
Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay
Sija De Gendt, Fred Shapiro, Jelena Juras, Els Van Assche, Geert Maertens, and Erwin Sablon
Part III. Molecular Biological Characterization
Processing of Hepatitis B Virus Surface Proteins
Volker Bruss
Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus
Stephan Urban
Nucleoprotein Transport of HBV Capsid Particles
Michael Kann
Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian cells
Jie Li, Yanyan Zheng, Jinah Choi, and Jing-hsiung Ou
Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Fabien Zoulim, ShuPing Tong, and Christian Trépo
A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein
Chiaho Shih and Ta-Tung Thomas Yuan
Studying DHBV Polymerase by In Vitro Transcription and Translation
Jianming Hu
Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System
Lisa Lott, Lena Notvall, and Robert E. Lanford
Localization of Duck Hepatitis B Virus Polymerase Within Cells
Ermei Yao and John E. Tavis
Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice
Zhenming Xu and Jing-hsiung OuTranscriptional Control of Hepatitis B Virus
Anneke K. Raney
In Vitro Reconstitution of e-Dependent Duck Hepatitis B Virus Replication Initiation
Jürgen Beck and Michael Nassal
Hepatitis B Viruses: A Triple Threat for Malignant Transformation of Hepatocytes
Charles E. Rogler
Index

Summary

Despite the availability of an effective vaccine, there are still 400 million people, worldwide who are chronically infected with hepatitis B virus (HBV). For them, the vaccine, as currently applied, has no value. Given the possible consequences of HBV infection, the number of those chronically infected with HBV presents an enormous public health challenge. For example, the major etiology of hepatocellular carcinoma (HCC) is chronic infection with HBV. Although fifth in cancer incidence, worldwide, HCC/liver cancer is the third leading cause of cancer death. The high mortality as- ciated with HCC arises because the disease is often detected late and is unresponsive to treatment. The number of deaths caused by PHCC is expected to rise over the next 20 years. Those chronically infected with HBV have a life risk of death to HCC of between 10 and 25%. Even the limited efficacy of drugs for the treatment of chronic HBV helps underscore the point that this disease is responsive to therapy. Drugs that target the polymerase (e. g. , hepsera and lamivudine) and interferon alpha represent two distinct strategies and show that both conventional antiviral and immunothe- peutic approaches can be used in management. However, the current inventory of therapeutics is inadequate. Interferon alpha is of limited value, only parenterally ava- able, and fraught with adverse reactions.

Additional text

From the reviews:

"Both volumes provide an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D. A major attribute of the volumes is that as they cover a wide range of subjects, the reader has the opportunity to access information they would not normally encounter. Volumes 1 and 2 are an excellent reference source and the methodologies described present the opportunity for both new and experienced researchers to study the molecular aspects of HBV and HDV infection."-SGM Quarterly

"...an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D...." - Microbiology Today

"The book is an extremely comprehensive collection of valuable modern protocols on detection of HBV DNA in serum and liver … . In summary, the general layout of the book is of excellent quality. Details are painstakingly provided so that sophisticated readers can profit from first hand top quality information derived from people who have personally worked at the bench. The substantial number of references will also allow easy access to the specific literature in the field." (M. Mondelli, Digestive and Liver Disease, Vol. 37, 2005)

Report

From the reviews: "Both volumes provide an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D. A major attribute of the volumes is that as they cover a wide range of subjects, the reader has the opportunity to access information they would not normally encounter. Volumes 1 and 2 are an excellent reference source and the methodologies described present the opportunity for both new and experienced researchers to study the molecular aspects of HBV and HDV infection."-SGM Quarterly "...an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D...." - Microbiology Today "The book is an extremely comprehensive collection of valuable modern protocols on detection of HBV DNA in serum and liver . In summary, the general layout of the book is of excellent quality. Details are painstakingly provided so that sophisticated readers can profit from first hand top quality information derived from people who have personally worked at the bench. The substantial number of references will also allow easy access to the specific literature in the field." (M. Mondelli, Digestive and Liver Disease, Vol. 37, 2005)

Product details

• Assisted by: Robert K. Hamatake (Editor), Johnson Y. N. Lau (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 01.01.2004
• EAN: 9781588291059
• ISBN: 978-1-58829-105-9
• No. of pages: 376
• Weight: 1 g
• Illustrations: 109 SW-Abb., 1 Farbabb., 37 Duoton-Abb., 72 SW-Zeichn.
• Series: Methods in Molecular Medicine; Methods in Molecular Medicine
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Non-clinical medicine; B, Medicine, Diseases, infectious diseases