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This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural natural products. Chapters guide readers through protocols on heterologous expression techniques, gene disruption, modified pathway regulators, and in-vitro studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Engineering Natural Product Biosynthesis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Chapter 13 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
List of contents
A bioinformatics workflow for investigating fungal biosynthetic gene clusters.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression - Aspergillus oryzae as a heterologous host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host.- Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans using episomal vectors.- Targeted Genetic Engineering via Agrobacterium-mediated transformation in Fusarium solani.- Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host.- Evolutionary genome mining for the discovery and engineering of natural product biosynthesis.- Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters.- Engineering modular polyketide biosynthesis inStreptomyces using CRISPR/Cas: a practical guide.- CRISPR/Cas9-Based Methods for Inactivating Actinobacterial Biosynthetic Genes and Elucidating Function.- Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in Streptomyces.- Heterologous expression, purification, and characterization of type II polyketide acyl carrier proteins.- Cyanobacterial Genome Sequencing, Annotation and Bioinformatics.- Single crossover to inactivate target gene in cyanobacteria.- Double crossover approach to inactivate target gene in cyanobacteria.- Expression of Cyanobacterial Biosynthetic Gene Clusters in Escherichia coli.- Saccharomyces cerevisiae as a heterologous host for natural products.- Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host.- Rapid Combinatorial Co-Expression of Biosynthetic Genes by Transient Expression in the Plant Host Nicotiana benthamiana.- Optimized Tools and Methods for Methanotroph Genome Editing.- Microarray-based screening of putative Hsp90 inhibitors predicted, and isolated from microorganisms.- Isolation of Water-Soluble Metabolites from Marine Invertebrates and Microorganisms.- Natural Product Investigation in Lichens: Extraction and HPLC analysis of Secondary Compounds in Mycobiont Cultures.
