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This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols.
Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.
List of contents
Extraction and Analysis of Phenolic Compounds From Grape Berries.- Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli.- Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings.- A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja.- Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles.- Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential.- Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses.- Visualization of Suberization and Lignification in Sugarcane.- Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species.- Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS.- Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes.- Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging.- Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants.- Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa.- Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants.- Isolation of specialized plant cells by fluorescence-activated cell sorting.- X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts.- Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae.- Methods of mimosine extraction fromLeucaena leucocephala (Lam.) de Wit leaves.- Microbial production, extraction, and quantitative analysis of isoprenoids.
