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This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
List of contents
Working safely with African swine fever virus.- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus.- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope.- Primary macrophage culture from porcine blood and lungs.- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses.- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction.- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks.- Genotyping of African swine fever virus.- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag).- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS).- African Swine Fever Virus Haemadosrption Inhibition Assay.- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-Gamma) producing cells.- Purification of African swine fever virus.- African swine fever virus plaque assay and disinfectant testing.- Bioorthogonal labelling of African swine fever virus infected cells.- Whole genome sequencing of African swine fever virus.
