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This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.
List of contents
Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria.- Purification of Primary Decidual Natural Killer Cells for Functional Analysis.- In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells.- Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells.- Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease.- Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq.- Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses.- Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells.- Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes.- Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis.- Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling.- Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer.- Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors.- Method to Study Adaptive NK Cells Following MCMV Infections.- Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains.- NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay.- Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions.- In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons.- CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells.- Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine.- Gene Transductionof Natural Killer Cells for Clinical Application.
