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This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.
List of contents
Recent Advances in Plant Gene Silencing Methods.- Strategies for Efficient RNAi Based Gene Silencing of Viral Genes For Disease Resistance In Plants.- Genome Editing and Designer Crops for the Future.- In Silico Methods for the identification of Viral-derived Small interfering RNAs (vsiRNAs) and their application in Plant genomics.- Barley stripe mosaic virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes In Wheat And Barley.-Virus-induced Gene Silencing in Wheat and Related Monocot Species.- Virus-Induced Gene Silencing in Sorghum using Brome mosaic virus.- RTBV-based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications.- Optimization of Tobacco rattle virus (TRV)-based Virus-Induced Gene Silencing (VIGS) in Tomato.- Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants.- VIGS-based Gene Silencing for Assessing Mineral Nutrient Acquisition.- High throughput Analysis of Gene Function Under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants.- A Method for Developing RNAi-derived Resistance in Cowpea Against Geminiviruses.- In vitro method for synthesis of large scale ds RNA molecule as a Novel Plant Protection Strategy.- Fine-tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs.- Trans-Kingdom RNA silencing in Plant-Fungal Disease Control.- An integrated bioinformatics and functional approach for miRNA validation.- Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants.- Multigene Transformation Through Cre-lox mediated Site-Specific Integration in Rice.- An Improvised Hairy Root Transformation Method for Efficient Gene Silencing in Roots and Nodules of Arachis hypogaea.- A method to reduce off-targets in CRISPR/Cas9 system in plants.
