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A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
List of contents
Establishment and Maintenance of Normal Human Keratinocyte Cultures.- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters.- Isolation and Culture of Human Osteoblasts.- Human Osteoclast Culture From Peripheral Blood Monocytes.- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation.- Human Myoblasts and Muscle-Derived SP Cells.- Cell Cultures of Autopsy-Derived Fibroblasts.- Primary Culture and Differentiation of Human Adipocyte Precursor Cells.- Human Mononuclear Phagocytes in Tissue Culture.- Purification of Peripheral Blood Natural Killer Cells.- Human Fetal Brain Cell Culture.- Culturing Human Schwann Cells.- Well-Differentiated Human Airway Epithelial Cell Cultures.- Isolation and Culture of Human Alveolar Epithelial Cells.- A New Approach to Primary Culture of Human Gastric Epithelium.- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach.- Isolation and Culture of Human Hepatocytes.- Glomerular Epithelial and Mesangial Cell Culture and Characterization.- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules.- Culture of Parathyroid Cells.- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media.- Primary Culture of Human Antral Endocrine and Epithelial Cells.- Conjunctiva Organ and Cell Culture.- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.
Summary
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Additional text
"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function
"...a very useful general text for researchers involved in human cell cultures. -Immunology News
From Reviews of the First Edition...
"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology
"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology
"...will be of significance to a very wide range of scientists." -ISSX Newsletter
"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology
"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter
Report
"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function
"...a very useful general text for researchers involved in human cell cultures. -Immunology News
From Reviews of the First Edition...
"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology
"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology
"...will be of significance to a very wide range of scientists." -ISSX Newsletter
"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology
"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter
