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This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.
List of contents
Heterologous production of plant terpenes in the photosynthetic bacterium Rhodobacter capsulatus.- Design of multiplexing CRISPR/Cas9 constructs for plant genome engineering using the GoldenBraid DNA assembly standard.- pSHDY - a new tool for genetic engineering of cyanobacteria.- Cas9-mediated targeted mutagenesis in plants.- Generation of stable, light-driven co-cultures of cyanobacteria with heterotrophic microbes.- Gene editing in green alga Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins.- Coexpression and reconstitution of enzymatic cascades in bacteria using UbiGate.- Homo-FRET imaging to study protein-protein interaction and complex formation in plants.- Plant X-tender toolbox for the assembly and expression of multiple transcriptional units in plants.- AGROBEST: a highly efficient Agrobacterium-mediated transient expression system in Arabidopsis seedlings.- Genetically-encoded biosensors for the quantitative analysis of auxin dynamics in plant cells.- Exploiting the Gal4/UAS system as plant orthogonal molecular toolbox to control reporter expression in Arabidopsis protoplasts.- Mathematical Modelling in Plant Synthetic Biology.- In vivo Epitope-tagging of Plant Mitochondria.- Engineering destabilizing N termini in plastids.- Trichome transcripts as efficiency control for Synthetic Biology and Molecular Farming.
