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Zusatztext "The topics are well illustrated and range from fields of biochemistry! molecular biology and biotechnology to analytical chemistry! pharmaceutical and environmental science. This handbook can be an excellent source of information not only to the students but also to the persons involved in research and academia." -Carbohydrate Polymers Informationen zum Autor Hage\! David S.; Cazes\! Jack Klappentext This essential handbook guides researchers in the theory! applications! and practical use of affinity chromatography. It explores preparative and analytical applications of affinity methods as well as recent developments in the use of affinity chromatography with other separation techniques and in combination with biosensors! mass spectometry! or microanalytical systems. This edition has been extensively revised and features new and expanded discussions on topics such as new ligands! recently developed applications! and affinity-based immunossays. It also discusses applications ranging from clinical and pharmaceutical analysis to biotechnology and environmental testing. Zusammenfassung A guide to the theory! applications! and practical use of affinity chromatography. It explores preparative and analytical applications of affinity methods as well as the developments in the use of affinity chromatography with other separation techniques and in combination with biosensors! mass spectrometry! or microanalytical systems. Inhaltsverzeichnis INTRODUCTION AND BASIC CONCEPTS An Introduction to Affinity Chromatography; D.S. Hage and P.F. Ruhn Support Materials for Affinity Chromatography; P. Gustavsson and P. Larsson Immobilization Methods for Affinity Chromatography; H.S. Kim and D.S. Hage Application and Elution in Affinity Chromatography; D.S. Hage! H. Xuan! and M.A. Nelson GENERAL AFFINITY LIGANDS AND METHODS Bioaffinity Chromatography; D.S. Hage! M. Bian! R. Burks! E. Karle! C. Ohnmacht! and C. Wa Immunoaffinity Chromatography; D.S. Hage and T.M. Phillips DNA Affinity Chromatography; R.A. Moxley! S. Oak! H. Gadgil! and H.W. Jarrett Boronate Affinity Chromatography; X. Liu and W.H. Scouten Dye-Ligand and Biomimetic Affinity Chromatography; N.E. Labrou! K. Mazitsos! and Y.D. Clonis Immobilized Metal-Ion Affinity Chromatography; D. Todorova and M.A. Vijayalakshmi PREPARATIVE APPLICATIONS General Considerations in Preparative Affinity Chromatography; A. Subramanian Affinity Chromatography of Enzymes; F. Friedberg and A.R. Rhoads Isolation of Recombinant Proteins by Affinity Chromatography; A. Subramanian Affinity Chromatography in Antibody and Antigen Purification; T.M. Phillips Affinity Chromatography of Regulatory and Signal-Transducing Proteins; A.R. Rhoads and F. Friedberg Receptor-Affinity Chromatography; P. Bailon! M. Nachman-Clewner! C. L. Spence! and G.K. Ehrlich ANALYTICAL AND SEMIPREPARATIVE APPLICATIONS Affinity Methods in Clinical and Pharmaceutical Analysis; Carrie A.C. Wolfe! W. Clarke and D.S. Hage Affinity Chromatography in Biotechnology; N. Jordan and I. Krull Environmental Analysis by Affinity Chromatography; M.A. Nelson and D.S. Hage Affinity Chromatography in Molecular Biology; L.A. Jurado! S.Oak! Himanshu Gadgil! R.A. Moxley! and H.W. Jarrett Affinity-Based Chiral Stationary Phases; S. Patel! I.W. Wainer and W.J. Lough BIOPHYSICAL APPLICATIONS Quantitative Affinity Chromatography: Practical Aspects; D.S. Hage and J. Chen Quantitative Affinity Chromatography: Recent Theoretical Developments; D.J. Winzor Chromatographic Studies of Molecular Recognition and Solute Binding to Enzymes and Plasma Proteins; S. Patel! I.W. Wainer and W.J. Lough Affinity-Based Optical Biosensors; S.D. Long and D.G. Myszka RECENT DEVELOPMENTS Affinity Ligands in Capillary Electrophoresis; Niels H. H. Heegaard and C. Schou Affinity Mass Spectrometry; C.J. Briscoe! W. Clarke and D.S. Hage Microanalytical Methods Based ...
