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This volume presents an all-inclusive overview of molecular biological methods to study ABA signaling transport, and metabolism in several plant species. The chapters in this book cover topics such as biochemical and molecular biological characterization of ABA receptors; assay systems for ABA transporters; quantification and visualization of ABA levels; and comprehensive omics approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Abscisic Acid: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this developing field.
List of contents
Inhibition of Type 2C Protein Phosphatases by ABA Receptors in Abscisic Acid-Mediated Plant Stress Responses.- Inhibition of SnRK2 Kinases by Type 2C Protein Phosphatases.- Reconstitution of the Core ABA Signaling in Protoplasts: Transcriptional Activators.- Affinity Purification of Ubiquitinated Proteins Using p62-Agarose to Assess Ubiquitination of Clade A PP2Cs.- Microscopic Imaging of Endosomal Trafficking of ABA Receptors.- ABA Transport Assay in Plant Single Cell System.- Screening of ABA Transporters by A Yeast Two-Hybrid System-Based Screening using the Receptor Complex as a Sensor.- Use of Micrografting to Study the Role Played by Peptide Signals in ABA Biosynthesis.- Gas Exchange Measurements in Systemic Signaling Studies.- Liquid Chromatography-Tandem Mass Spectrometry-Based Profiling of Plant Hormones.- Live-Imaging of Abscisic Acid Dynamics using Genetically Encoded Fluorescence Resonance Energy Transfer (FRET)-Based ABA Biosensors.- In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling.- Phosphoproteomic Approaches to Evaluate ABA Signaling.- Affinity Purification followed by Liquid Chromatography-Tandem Mass Spectrometry to Identify Proteins Interacting with ABA Signaling Components.- SWATH-MS Proteomic Approach to Discover Novel Protein Targets and Pathways in Response to Abscisic Acid.- Genome-Wide Association Analyses to Identify SNPs Related to Drought Tolerance.- Chemical Approaches for Improving Plant Water Use.
