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This volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.
List of contents
Childhood Medulloblastoma: An Overview.- Real-time PCR assays on formalin-fixed paraffin embedded medulloblastomas.- Validation of microRNA target genes using luciferase reporter assay and western blot analysis.- Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY.- Quantification of microRNAs in cells and tissues using stem-loop RT PCR and qPCR.- Analysis of Microarray Data from Medulloblastoma tissue samples.- Advancement of single- cell sequencing in Medulloblastoma.- Flow cytometry approaches to obtain medulloblastoma stem cells from bulk cultures.- Drug Screening assays on Medulloblastoma Stem Cells using compound libraries.- 3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents.- In vitro screening assay for activators of T- cell migration to solid tumors.- Impact of Nano preparation of phytoconstituents in Medulloblastoma.- Challengesand Recent Advances of novel chemical inhibitors in Medulloblastoma Therapy.- Role of ayurvedic plants as anti-cancer agents.- In vivo tumorigenicity assays using subcutaneous and orthotopic xenografts in immunodeficient mice.- Manufacture of an Oncolytic Measles Virus-Sensitive Immune-Competent Mouse Model of Medulloblastoma.- Preclinical Research Strategy Development for RNAi-based therapies in Oncology using patient-centred information from public databases of Human Protein Atlas and R2Genomics Platform.- Magnetic resonance imaging in the contemporary management of medulloblastoma: current and emerging applications.- Multidisciplinary Management of Medulloblastoma: Consensus, Challenges and Controversies.
