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In the present in vitro study, Antimicrobial potential of Alum as well as its Bhasma- Sphatika Bhasma was evaluated against total 12 organisms, viz., 6 Gram Negative, 4 Gram Positive and 2 Fungi using various assays such as MIC, MBC/MFC and Agar Well Diffusion method. Alum as well as Sphatika Bhasma revealed broad spectrum Antibacterial activities inhibiting gram positive as well as gram negative organisms and moderate antifungal activities. Hence, Antibiofilm potential of Alum and Sphatika Bhasma was evaluated against total 10 bacterial biofilms using crystal violet staining method. Antibiofilm effect of Alum was found to be in the order of S. lutea > P. aeruginosa > E. coli > MRSA > S. typhi > B. subtilis > K. pneumoniae > S. flexneri > E. faecalis > P. vulgaris, whereas, Antibiofilm activity of Sphatika Bhasma was noted in the order of P. aeruginosa > S. lutea > E. coli > K. pneumoniae > S. typhi > P. vulgaris > S. flexneri > MRSA > B. subtilis > E. faecalis. Antimicrobial, Antibiofilm and Antioxidant effects of Alum and Sphatika Bhasma could be attributed to their Phenolic and Flavonoid contents as well as presence of elements such as Al, Ca, Cu, Fe, K, Mg, Na and Zn.
About the author
Dr. Anuya Aparna Rege has completed her post doctoral study at Haffkine Institute, Mumbai. She has obtained her Ph.D. in Microbiology from University of Mumbai. Her area of interests are microbiology & natural products. She has publications in reputed journals. Currently she is working as a senior scientific officer at Haffkine Institute.
