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This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians' toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Clinical Proteomics: Methods and Protocols serves as an ideal guide for researchers working to expand upon the knowledge base needed to push forward toward a more personalized version of medicine.
List of contents
Bile Processing Protocol for Improved Proteomic Analysis.- MS-Based Extracellular Vesicles (EVs) Analysis: An Application to Helminth-Secreted EVs.- Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry.- Data-Independent Acquisition Mass Spectrometry-Based Deep Proteome Analysis for Hydrophobic Proteins from Dried Blood Spots Enriched by Sodium Carbonate Precipitation.- Sample Processing for Metaproteomic Analysis of Human Gut Microbiota.- Mass Spectrometry-Based Analytical Strategy for Single Cell Proteomics.- Lysine Acetylation Stoichiometry Analysis at the Proteome Level.- Implementation of Clinical Phosphoproteomics and Proteomics for Personalized Medicine.- pH/Acetonitrile-Gradient-Reversed-Phase Fractionation of Enriched Hyper-Citrullinated Library in Combination with LC-MS/MS Analysis for Confident Identification of Citrullinated Peptides.- Purification of HLA Immunopeptidomes from Human Thymus.- Generation of HLA Allele-Specific Spectral Libraries to Identify and Quantify Immunopeptidomes by SWATH/DIA-MS.- Immunopeptidomic Analysis of the Phosphopeptidome Displayed by Hla Class I Molecules.- Development of a Standardized MRM Method for Quantification of One Carbon Metabolism Enzymes.- Molecular Histology Analysis of Cryopreserved Tissue Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS).- The Human Protein Atlas and Antibody-Based Tissue Profiling in Clinical Proteomics.- Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS.- Combining Electron Microscopy (EM) and Cross-Linking Mass Spectrometry (XL-MS) for Structural Characterization of Protein Complexes.- Accurate Prediction of Protein Sequences for Proteogenomics Data Integration.
