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This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches.
List of contents
Clinical Staging of Ovarian Cancer.- Pathologic Classification of Ovarian Cancer.- Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer.- Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors.- Processing and Analysis of Ascites.- Multispectral Staining and Analysis of Extracellular Matrix.- Quantitative Analysis of the Extracellular Matrix by Immunoblot.- Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity.- Culturing Primary Human Mesothelial Cells.- Isolation of Normal and Cancer-Associated Fibroblasts.- Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum.- Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF).- Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer.- In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer.- The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods.- Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection.- Humanized Patient-Derived Xenograft Models of Ovarian Cancer.- Xenograft Models of Ovarian Cancer for Therapy Evaluation.- Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer.
