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This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field
List of contents
Introduction to Immunohistochemistry. From Evolving Science to Timeless Art.- Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present.- Sample Preparation & Fixation for Histology and Pathology.- Tissue Processing.- Antigen Retrieval and Signal Amplification.- Primary Antibody Selection and Blocking Techniques for Immunohistochemistry.- Probes for Fluorescent Visualization of Specific Cellular Organelles.- Enzymatic Immunohistochemistry.- Fluorescent Immunohistochemistry.- Double Labeling Fluorescent Immunocytochemistry.- Multiplexing and Spectral Microscopy.- In situ Hybridization (Fluorescent and Enzymatic).- Proximity Ligation Assay (PLA).- Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System.- Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres.- ER+ Breast Cancer Mammosphere Formation and Analysis.- Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry.- Comet Assay for the Detection of Single and Double Strand DNA Breaks.- Fluorescent-Activated Cell Sorting (Flow Cytometry).
