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For both volumes:
Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
List of contents
BAC Library Construction.- Construction of Small Genome BAC Library for Functional and Genomic Applications.- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging.- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions.- Purification of BAC DNA.- Hybridization-Based Selection of BAC Clones.- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction.- BAC Mapping Using Fluorescence In Situ Hybridization.- High-Throughput BAC Fingerprinting.- BAC End Sequencing.- Radiation Hybrid Mapping With BAC Ends.- Shotgun Library Construction for DNA Sequencing.- Rolling Circle Amplification for Sequencing Templates.- Transposon-Mediated Sequencing.- Pyrosequencing.- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions.- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions.- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers.- BAC Finishing Strategies.- Using the TIGR Assembler in Shotgun Sequencing Projects.- Finishing "Working Draft" BAC Projects by Directed Sequencing With ThermoFidelase and Fimers.- Optimized Multiplex Polymerase Chain Reaction.- Assembly of DNA Sequencing Data.- Sequence Finishing.- Quality Assessment of Finished BAC Sequences.
Summary
For both volumes:
Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
