Protein Purification - Design and Scale Up of Downstream Processing

A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables.

List of contents

Process Design and Competitive Advantage.

Strategies for Process Design.

Protein Characteristics.

Analytical Methods.

Impact of Upstream Operations.

Cell Disruption.

Centrifugation.

Precipitation and Extraction.

Filtration.

General Chromatography.

Ion Exchange Chromatography.

Hydrophobic Interaction Chromatography and Reversed PhaseChromatography.

Affinity Chromatography.

Gel Filtration Chromatography.

Large-Scale Examples.

Index.

About the author

Scott M. Wheelwright is the author of Protein Purification: Design and Scale up of Downstream Processing, published by Wiley.

Summary

A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes.

Product details

• Authors: Wheelwright, Scott M Wheelwright, Scott M. Wheelwright, Sm Wheelwright, WHEELWRIGHT SCOTT M, Wheelwright Scott M.
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 23.05.1994
• EAN: 9780471037231
• ISBN: 978-0-471-03723-1
• No. of pages: 244
• Subjects: Humanities, art, music > Linguistics and literary studies > Other languages / Other literatures; Natural sciences, medicine, IT, technology > Chemistry; Social sciences, law, business > Business > Economics; SCIENCE / Life Sciences / Biology, SCIENCE / Life Sciences / Biochemistry, SCIENCE / Chemistry / Organic, SCIENCE / Applied Sciences, TECHNOLOGY & ENGINEERING / Research, biochemistry, proteins, Polymer chemistry, Chemical biology