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This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.
List of contents
Methods to Visualize the Actin Cytoskeleton during Plant Cell Division.- Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana.- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips.- Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures.- Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission.- Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy.- Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells.- Kinematic Growth Analysis of Rice (Oryza sativa) Leaf.- Studying Cell Division Plane Positioning in Early-Stage Embryos.- Means to Quantify Vascular Cell File Numbers in Different Tissues.- Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis.- Quantification of Cell Division Angles in the Arabidopsis Root.- A Quantitative Method for Evaluating Phragmoplast Morphology.- Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis.- Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells.- Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae.
