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This detailed volume presents a variety of specialized analytical methods on a wide range of glycolipids. From sphingoglycolipids to lipid A, an immune system stimulant present in lipopolysaccharide, the book delves into how glycolipid structure on the surface of the cell membrane, which characterizes each organism, is crucial for the coexistence or repulsion between organisms. In addition, glycolipids play an important role in infections in animals and other organisms by targeting host cell membranes, if enveloped viruses are considered as living organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glycolipids: Methods and Protocols serves as an ideal guide to themost advanced glycolipid research in an effort to help researchers make their research more fruitful.
List of contents
Immunomodulatory Functions of -GalCer and a Derivative, -Carba-GalCer.- Chemical Synthesis and Molecular Interaction Analysis of a-Galactosyl Ceramide Derivatives as CD1d Ligands.- Immunomodulatory Functions of Glycolipids from Pathogens.- Chemical Synthesis and Immunomodulatory Functions of Bacterial Lipid As.- Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines.- Chemical Synthesis of Phosphatidylglucoside.- Sialidase-Resistant Ganglioside GM3 Analogues: Evaluation of Biological Activity.- Chemical Synthesis of Gangliosides.- Sialyltransferase Activity Assay for Ganglioside GM3 Synthase.- Construction of Sphingolipid Remodeled Cells by Genome Editing.- Mass Spectrometry of Neutral Glycosphingolipids.- Structural Analyses of the Glycolipids in Lipid Rafts.- Extraction, Purification, and Chemical Degradation of LPS from Gut Microbiota Strains.- NMR Analysis of Mammalian Glycolipids.- Distribution of Glycolipids in the Plasma Membrane Monitored by Specific Probes inCombination with Sodium Dodecyl Sulfate-Digested Freeze-Fracture Replica Labeling (SDS-FRL).- Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes.- Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes.- Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi.- Molecular Dynamics of Glycolipids in Liposomes.- Inhibitors of Glucosylceramide Synthase.- Comprehensive Glycan Analysis of Sphingolipids in Human Serum/Plasma.
