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This volume provides a comprehensive collection of methods for plant breeders and researchers working in functional genomics of cereal crops. Chapters detail advances in sequencing of cereal genomes, methods of traditional plant breeding, use of machine learning for genomic selection, random and targeted mutagenesis with CRISPR/Cas9, quantitative proteomics and phenotyping in cereals.
Authoritative and cutting-edge, Accelerated Breeding of Cereal Crops aims to be of interest to plant breeders, researchers, postdoctoral fellows, and students working in functional genomics for the development of the next generation of crop plants.
List of contents
Recent Advances In Sequencing of Cereal Genomes.- Mapping Quantitative Trait Loci in Wheat: Historic Perspective, Tools, and Methods For Analysis.- Introduction to Marker-Assisted Selection in Wheat Breeding.- Genome-wide Association Studies (GWAS) in Cereals.- ropGBM: An Ultra-Efficient Machine Learning Toolbox For Genomic Selection-Assisted Breeding In Crops.- Doubled Haploid Production through Microspore Culture.- Chemical and Physical Mutagenesis Approaches for Identification of Herbicide and Drought Tolerance Traits in Wheat.- Using TILLING Lines for Functional Genomics In Wheat.- A Transposon-Based Activation Tag System for Functional Genomics in Cereals: Detection Of Mping-Based Activation Tag Mobilization In Wheat.- A Modular Cloning Toolkit for Genome Editing in Cereals.- Genome Editing and Identification of Targeted Heritable Mutations in Wheat.- A Genotyping Protocol To Identify CRISPR/Cas9-edited Events in Hexaploid Wheat.- qPCR Methods for the Quantification Of Transgene Insert Copy Number and zygosity using the Comparative Ct method in transgenic Sorghum bicolor L. Moench.- A Roadmap for Undertaking Quantitative Proteomics in Plants: New Opportunities for Cereal Crops.- DNA Affinity Purification Sequencing (DAP-seq) for Mapping Genome-Wide Transcription Factor Binding Sites in Plants.- Basics of Sensor-based Phenotyping in Wheat.
