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Zusatztext "The book includes a short subject and a thorough list of references at the end of each chapter." -J. Am. Chem. Soc.! 2006 Informationen zum Autor Brian K. Nunnally Klappentext Since the early days of radioisotope sequencing! a wide variety of new DNA sequencing techniques have emerged. New methods are aimed at reducing the amount of sample needed! improving the accuracy! and reducing the amount of time needed to generate a sequence. This text discusses the different analytical DNA sequencing techniques and the exciting applications of DNA sequencing in areas such as forensics! the sequencing of ancient DNA! and genome sequencing. Advances in DNA separations using advanced capillary electrophoresis and microchip sequencing are presented. Chapters cover improvements in the detection of DNA sequencing fragments! the use of mass spectrometry! and single molecule detection. Zusammenfassung Offers a look at the DNA sequencing techniques that were developed and put to use during the Human Genome Project. This work traces the development of this branch of research since the early days of radioisotope sequencing. It describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. Inhaltsverzeichnis Introduction to DNA Sequencing: Sanger and Beyond . Developments in the Detection of DNA Sequencing Fragments Using Fluorescence: Energy Transfer and Fluorescence Lifetimes . Microscale Sample Preparation for DNA Sequencing and Genotyping . Microfabricated DNA Sequencing Devices. Analysis of Nucleic Acids by Mass Spectrometry . Sequencing the Single DNA Molecule. DNA Sequencing for Genome Analysis. Sequence Reconstruction from Nucleic-Acid Micro-Array Data . Ancient DNA. Forensic DNA Sequencing.
