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This updated and expanded edition explores key methodologies to study the fascinating phenomenon of how plants readjust their growth toward gravity. In addition to the protocols delivering broad applications for gaining insight into other plant physiological processes, this new volume also focuses on techniques involving plants in space or the use of microgravity analogs to study plant biological phenomenon. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Gravitropism: Methods and Protocols, Second Edition serves as an ideal guide for researchers studying the cellular, molecular, and biochemical networks that plants use to translate environmental stimuli into a growth response.
List of contents
Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds.- Evaluation of Gravitropism in Non-Seed Plants.- Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii.- A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates.- Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms.- Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants.- High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot.- Staging of Emerged Lateral Roots in Arabidopsis thaliana.- Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species.- The Analysis of Gravitropic Setpoint Angle Control in Plants.- A Flat Embedding Method to Orient Gravistimulated Root Samples for Sectioning.- Conducting Plant Experiments in Space and on the Moon.- Plant Proteomic Data Acquisition and Data Analyses: Lessons from Spaceflight.- Blueprints for Constructing Microgravity Analogs.- Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies.- Use of Reduced Gravity Simulators for Plant Biological Studies.- Understanding the Mechanisms of Gravity Resistance in Plants.- NASA's Ground-Based Microgravity Simulation Facility.- Evaluating the Effects of the Circadian Clock and Time of Day on Plant Gravitropic Responses.
