Biochemical Pathways and Environmental Responses in Plants: Part a

Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more.
Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.

List of contents

1. Structure, function, and engineering of plant polyketide synthases
Ikuro Abe
2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate?synthase involved in glucosinolate side-chain elongation
N Bisht
3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay
Sanja Roje
4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry
Haijun Liu
5. Biochemical characterization of plant aromatic aminotransferases
Hiroshi Maeda
6. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites
Soon Goo Lee
7. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions
Cynthia Holland
8. Plant metacaspase: an example of microcrystal structure determination and analysis
Qun Liu
9. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners
Chang-Jun Liu
10. Dirigent Protein Family Function and Structure
Norman G. Lewis
11. Lignin/Lignan Biosynthesis Structure/Function
Norman G. Lewis
12. Discovery and characterization of BAHD acylsugar acyltransferases
Robert L. Last
13. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent
Michael Sullivan
14. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta
Markus Wirtz
15. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo
Jeffrey Cameron
16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry
Peter Facchini
17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry
Kazuki Saito and Tetsuya Mori
18. Isoprene measurements to assess the methylerythritol pathway
Thomas D. Sharkey
19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry
Peter Dörmann and Katharina Gutbrod
20. Using targeted metabolomics to elucidate the indole auxin network in plants
Jerry D. Cohen and Adrian D. Hegeman
21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation
Ivo Feussner
22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes
Kirstin Feussner
23. Using stable isotopes and LC-MS for precursor of origin determination in plants
Clint Chapple
24. Measurement of flux through sulfate assimilation using [35S]sulfate
Stanislav Kopriva
25. Assessment of the activity of sulfate uptake and assimilation in plants
Rüdiger Hell
26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species
Philip Bates
27. Plant sphingolipid analysis
Jennifer E. Markham
28. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon
Joshua R. Widhalm
29. Controlled environments for highly reproducible cannabis 'omics' studies
Bernd Markus Lange
30. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis
Bernd Markus Lange
31. Systems and strategies for plant protein expression
Thomas D. Niehaus
32. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic

About the author

Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engineering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.

Product details

• Assisted by: Joseph Jez (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Released: 21.10.2022
• EAN: 9780323955614
• ISBN: 978-0-323-95561-4
• No. of pages: 459
• Series: Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; SCIENCE / Life Sciences / Biochemistry, SCIENCE / Life Sciences / Molecular Biology, biochemistry, molecular biology