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This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.
List of contents
Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling.- Generating Monocyte Derived Endothelial-Like Cells for Vascular Regeneration.- Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells.- On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix Scaffold under Mechanical Stimulations for Vascular Tissue Engineering.- End-Point Immobilization of Heparin on Electrospun Polycarbonate-Urethane Vascular Graft.- Microfluidic Co-Axial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits.- In Situ Fabrication and Perfusion of Tissue Engineered Blood Vessel Microphysiological System.- Peritoneal Pre-Conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch.- Fabrication of a Completely Biological and Anisotropic Human Mesenchymal Stem Cell-Based Vascular Graft.- Engineering Vascular Grafts with Multiphase Structures.- Fabrication of Small Diameter Tubular Grafts for Vascular Tissue Engineering Applications using Mulberry and Non-Mulberry Silk Proteins.- Fabrication and Evaluation of Tissue-Engineered Vascular Grafts with Hybrid Fibrous Structure.- Controlling Pore Size of Electrospun Vascular Grafts by Electrospraying of Poly(Ethylene Oxide) Microparticles.- Injectable Hydrogels for Vascular Tissue Engineering.- Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis.- Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners.- Vascular Graft Implantation using a Bilateral End-to-Side Aortoiliac Preclinical Model.- Quantification of In Vitro Blood-Brain Barrier Permeability.- Subcellular Force Quantification of Endothelial Cells using Silicone Pillar-Arrays.- Computational Assessment of Hemodynamics Vortices within the Cerebral Vasculature using Information Entropy.
