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Informationen zum Autor Deceased: February 12, 2017 Klappentext - Provides general guidelines and strategies for isolation and crystallization of membrane proteins- Gives detailed protocols that have wide application, and low specialized equipment needs- Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitopetagged proteins Zusammenfassung Intended for bench scientists working in the fields of biochemistry! biology! and proteomic research! this guide presents isolation and crystallization techniques! emphasizing the critical aspects unique to membrane proteins. It also explains the principles of the methods and provides protocols of general use. Inhaltsverzeichnis I. Strategies and Techniques Purification Strategies for Membrane Proteins Chromatographic Techniques and Basic Operations in Membrane Protein Purification Production and purification of recombinant membrane proteins SDS Electrophoresis Techniques Blue-Native Electrophoresis Preparative Isoelectric Focussing Membrane protein crystallization II. Discussion of Selected Isolation Protocols Lipid Dependent Inactivation and Reactivation of Bovine complex III Purification of affinity-epitope tagged G-protein coupled receptors Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D Crystallization Purification of the yeast cytochrome bc1 complex III. Crystallization of Membrane Proteins Antibody fragment-mediated crystallization of membrane proteins Crystallization of Wolinella succinogenes quinol:fumarate reductase Ba3-type cytochrome-c oxidase from Thermus thermophilus purification, crystallization, and crystal transformation Two-dimensional crystallization of membrane proteins: a practical guide In cubo crystallization of membrane proteins
