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This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Peptide Conjugation: Methodsand Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field.
List of contents
Peptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Conjugation of Peptides to Gold Nanoparticles.- Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus.- Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides.- Modification of Nanoparticles with Transferrin for Targeting Brain Tissues.- Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition.- Vitamin B12 - Peptide Nucleic Acid Conjugates.- Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides.- Solid-State, Thermal Synthesis of Peptide/Protein-Boron Cluster Conjugates.- Bioconjugation of Peptides to Hybrid Gold Nanoparticles.- Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2.- Electrochemically-Enabled C-Terminal Peptide Modifications.- Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates.- Chemical Protein Synthesis by Chemoselective ¿-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline.- Site-Specific Modification of Single-Chain Affinity Ligands for Fluorescence Labeling, Radiolabeling, and Bioconjugation.- Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags.- The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library.- The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation.- Synthesis of Lipopeptides by CLipPA Chemistry.- Constraining TAT Peptide by ¿PNA Hairpin for Enhanced Cellular Delivery of Biomolecules.- Preparation of mRNA Polyplexes with Post-Conjugated Endosome-Disruptive Peptides.- Targeted Subcellular Protein Delivery Using Cleavable CyclicCell-Penetrating Peptide-Conjugates.- Facile Chemoselective Modification of Thio-Ethers Generates Chiral Center-Induced Helical Peptides.
