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This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.
List of contents
Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method.- Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample.- RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells.- Preparation of Neonatal Rat Papillary Muscles for Contractile Studies.- In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine.- Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells.- Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining.- Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue.- Laser-induced Choroidal Neovascularization in Rats.- Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain.- Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.- Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues.- Visualization of Fundus Blood Vessels.- Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements.- Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery.- Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus.- Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.
