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This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.
List of contents
Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts.- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons.- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9.- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules.- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons.- Direct Conversion of Human Fibroblasts to Induced Neurons.- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts.- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs).- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors.- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons.- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression.- Transcriptional Profiling During Neural Conversion.- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo.- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods.- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions.- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders.- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.
