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This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.
List of contents
Making Cardiomyocytes from Pluripotent Stem Cells.- A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells.- Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture.- Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells.- Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches.- Fabrication of Cardiac Constructs using bio-3D Printer.- Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium.- Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method.- Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages.- Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes.- Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies.- Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.- Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique.- Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells.- Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis.- Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells.- A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues.- Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart.- RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes.- Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells.- Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs).- Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9.- CRISPRi/a Screening with Human iPSCs.- Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model.- Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey.
