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This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 1 explores general topics and transversal methods in DH technology; DH production in two alliaceae, onion, and leek; and DH production in cereals such as barley, durum, bread, oat, and japonica rice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Doubled Haploid Technology, Volume 1: General Topics, Alliaceae, Cereals is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.
List of contents
Overview of In Vitro and In Vivo Doubled Haploid Technologies.- Applications of Doubled Haploids in Plant Breeding and Applied Research.- Species with Haploid or Doubled Haploid Protocols.- Analysis of Ploidy in Haploids and Doubled Haploids.- Methods for Chromosome Doubling.- Doubled Haploid Onion (Allium cepa L.) Production via In Vitro Gynogenesis.- In Vitro Gynogenesis in Leek (Allium ampeloprasum L.).- Barley Isolated Microspore Culture.- Site-Directed Mutagenesis in Barley using RNA-GUided Cas Endonucleases during Microspore-Derived Generation of Doubled Haploids.- Generation of Doubled Haploid Barley by Interspecific Pollination with Hordeum bulbosum.- Bread Wheat Doubled Haploid Production by Anther Culture.- Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat.- In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat.- Production of Wheat Doubled Haploids through Intergeneric Hybridization with Maize.- Isolation of Staged and Viable Maize Microspores for DH Production.- Triticale Isolated Microspore Culture for Doubled Haploid Production.- Oat (Avena sativa L.) Anther Culture.- Oat Doubled Haploid Production through Wide Hybridization with Maize.- Anther Culture and Chromosome Doubling in Mediterranean Japonica Rice.- High Throughtput Doubled Haploid Production for Indica Rice Breeding.
