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This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 3 looks at the emerging tools used in DH technology; protocols based on anther culture, in vitro gynogenesis and hybridization with irradiated pollen for DH production in cucubits such as cucumber, melon, watermelon, and summer squash; techniques to produce haploids and DHs in different woody species and genera such as citrus, coconut, walnut, and almond; and methods for DH production in borage, Saintpaulia ionantha, cow cockle, and marigold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Doubled Haploid Technology, Volume 3: Emerging Tools, Cucurbits, Trees, Other Species is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.
List of contents
Centromere Engineering as an Emerging Tool for Haploid Plants Production: Advances and Challenges.- Fractional Factorial Designs: An Underutilized Tool for Rapid In Vitro System Development.- Applications of Impedance Flow Cytometry in Doubled Haploid Technology.- Production of Doubled Haploid Plants in Cucumber (Cucumis sativus L.) through Anther Culture.- Doubled Haploid Parthenogenetic Production of Melon 'Piel de Sapo'.- Doubled Haploid Production in Watermelon.- Anther Culture in Cucurbita Species.- Gynogenesis in Cucurbita Species.- Induction of Parthenogenesis by Irradiated Pollen in Cucurbita Species.- Microspore Embryogenesis in Citrus.- Anther Culture of Coconut (Cocos nucifera L.).- Production of Haploid and Doubled Haploid Lines in Nut Crops: Persian Walnut, Almond, and Hazelnut.- Production of Doubled Haploid Embryos from Cork Oak Anther Cultures by Antimitotic Agents and Temperature Stress.- Anther Culture in Jatropha curcas L. - A Tree Species.- Haploid Plant Production in Borage (Borago officinalis L.) by Anther Culture.- Anther-Based Regeneration of African Violet (Saintpaulia ionantha).- Doubled Haploidy for Cow Cockle (Saponaria Vaccaria L.).- Production of Haploids and Doubled Haploids in Marigold (Tagetes spp.) using Anther Culture.- Anther Culture of Chickpea (Cicer arietinum L.).- Production of Homozygous Red Beet (Beta vulgaris L subsp. vulgaris) Plants by Ovule-Culture.- Production of Doubled Haploid Sugar Beet (Beta vulgaris L.) Plants through Gynogenesis.
