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This detailed edition reflects the significant new findings in the components of permeability barriers and how they work in different tissues with a collection of cutting-edge techniques. Chapters explore the formation, maintenance, regulation, and dynamics of permeability barriers in an effort to push the boundaries of the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Permeability Barrier: Methods and Protocols, Second Edition serves as an invaluable guide for both experts but novices in the stem cell field and other related areas of research.
List of contents
- Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine. - Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line. - Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium. - Selective Regional Isolation of Brain Microvessels. - Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models. - In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing. - Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase. - Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo. - Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopyin Freely Moving Rats. - Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue. - Measurement of Airway Epithelial Permeability: Methods and Protocols. - Vascular Permeability Assays In Vivo. - Endothelial Permeability Assays In Vitro. - Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells. - An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity. - Ussing Chamber Methods to Study the Esophageal Epithelial Barrier. - A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher. - Differentiating Between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo. - Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly. - A Method to Prepare a Bioprobe for Regulatory Science of the Drug Delivery System to the Brain: An Angulin Binder to Modulate Tricellular Tight Junction-Seal. - dSTORM Imaging and Analysis of Desmosome Architecture. - Correction to: Differentiating Between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo.
