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This new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with in vitro and in vivo cytotoxicity assays, methods to detect senescent T cells, in vivo and in vitro models to understand immune and bone cells cross-talk, microscopy and in vivo imaging, as well as "Omics" approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and key implementation advice for best results in the lab.
Authoritative and up-to-date, Cytotoxic T-Cells: Methods and Protocols, Second Edition serves as an ideal guidefor researchers working with these vital cells.
List of contents
Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting.- Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells.- Tumor Infiltrating T-Cell Cytotoxicity Assay.- Antigen-Specific In Vivo Killing Assay.- CTL ELISPOT Assay and T-Cell Detection.- Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single Cell-RNA Sequencing.- In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells.- Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray.- A Review of Proteomics Strategies to Study T-Cells Activation and Function in Cancer Disease.- Methods to Study Post-Translational Modification Patterns in Cytotoxic T-Cells and Cancer.- Whole Exome Sequencing for the Identification of Mutations in Cd8+T-Cells.- Handling High-Throughput Omics Data for Systems Genetics Analysis.- Analysis of miRNA Expression Using Digital PCR and the QuantStudio(TM) 3D Digital PCR System.- Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview.- Protein-Bound Uremic Toxins and Immunity.- Gut Microbiota, the Immune System, and Cytotoxic T Lymphocytes.
