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This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves asan invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.
List of contents
Mass Spectrometry-Based Lipidomics: An Overview.- High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry.- Global Lipidomics Profiling by a High Resolution LC-MS Platform.- Comprehensive Structural Characterization of Lipids by Coupling Paternò-Büchi Reaction and Tandem Mass Spectrometry.- Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis.- Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics after Methylation.- A Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain Fatty Acids.- One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS.- Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument.- Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High Resolution Mass Spectrometry Platform.- Mass Spectrometric Analysis of Meibomian Gland Lipids.- Quantification of Plasma Oxylipins Using Solid Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry.- Targeted Lipidomics of Drosophila melanogaster During Development.- Shotgun Lipidomic Analysis of Leishmania Cells.- Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry.- Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi.- Analytical Methodologies for Lipidomics in Hemp Plant.- Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry.- Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-Photoionization Mass Spectrometry.- Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach.
