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Informationen zum Autor Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.
List of contents
1. Crosslinking immunoprecipitation of Upf1 in yeast
Kristian Baker
2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19
Robert Brosh
3. Bulk phase biochemistry of the Pif1 helicase
Matthew Bochman
4. Translational control by helicases during cellular stress
Tim Bolger
5. Measuring DDX3-dependent protein synthesis in vitro and in cells
Stephen Floor
6. Purification of Mtr4 and Mtr4-mediated complexes
Sean Johnson
7. Plan of attack for studying DEAD-box proteins
Katrin Karbstein
8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systems
Ailong Ke
9. Helicase mediated vectorial folding of G-quadruplex
Sua Myong
10. Detecting G4 quadruplex unwinding
Katrin Paeschke
11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopy
Anna Marie Pyle
12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteins
Rick Russell
13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA Helicases
Sheena D'Arcy
14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexes
Christopher D. Lima
15. Study of RNA helicases with magnetic tweezers
Herve Le Hir
16. Single-molecule FRET to analyze the dynamics of RNA helicases
Dagmar Klostermeier
17. Transcriptional regulation by a RecQ helicase
Sudha Sharma
18. In vitro characterization of the DEXH helicase Dhr1 from yeast
Arlen Johnson
