Carotenoids: Carotenoid and Apocarotenoid Analysis

Carotenoids: Carotenoid and Apocarotenoid Analysis, Volume 670, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume covering Getting to know carotenoids, Laser capture of tissues for micro-scale carotenoid analyses, Metabolic engineering of carotenoids: procedures for metabolomic characterization, LC-MS analysis of intracellular metabolites for precursors to the carotenoid pathway, Use of E. coli to produce carotenoid standards, HPLC analysis of carotenoids from Bacteria, Purification and development of standards for carotenoid quantification in plant tissues, and much more.
Additional sections in this release cover Ultra-High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Apocarotenoids, Detection and analysis of novel and known volatile plant apocarotenoids, Carotenoid extraction, detection, and analysis in citrus, Strategies For The Separation And Tentative Identification Of Geometrical (Cis/Trans, Z/E) Isomers Of Carotenoids, Use of stable isotopes to study bioconversion and bioefficacy of pro-vitamin A carotenoids, Carotenoid extraction and analysis of blood plasma/serum, and more.

List of contents

1. Getting to know carotenoids
George Britton
2. Laser capture of tissues for micro-scale carotenoid analyses
James Giovannoni
3. Metabolic engineering of carotenoids: procedures for metabolomic characterization
Paul David Fraser
4. LC-MS analysis of intracellular metabolites for precursors to the carotenoid pathway
Claudia Vickers
5. Use of E. coli to produce carotenoid standards
Norihiko Misawa, Miho Takemura and Takashi Maoka
6. HPLC analysis of carotenoids from Bacteria
Gerhard Sandmann
7. Purification and development of standards for carotenoid quantification in plant tissues
Christopher Ian Cazzonelli, Jwalit Nayak, Yagiz Alagoz and Sidra Anwar
8. Ultra-High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Apocarotenoids
Salim Al Babili, Jianing Mi, Yagiz Alagoz, Juan Camilo Moreno, Kit Xi Liew, Aparna Balakrishna and Xiongjie Zheng
9. Detection and analysis of novel and known volatile plant apocarotenoids
Barry James Pogson, Ryan McQuinn, Julie Leroux and Thy Thuc Truong
10. Carotenoid extraction, detection, and analysis in citrus
Xiuxin Deng, Kaijie Zhu, Xiongjie Zheng, Yun Liu, Hongyan Chen, Yingzi Zhang and Junli Ye
11. Strategies For The Separation And Tentative Identification Of Geometrical (Cis/Trans, Z/E) Isomers Of Carotenoids
Antonio J. Meléndez-Martínez
12. Use of stable isotopes to study bioconversion and bioefficacy of pro-vitamin A carotenoids
Georg Lietz
13. Carotenoid extraction and analysis of blood plasma/serum
Jessica L. Cooperstone and Maria Sholola
14. Methods for analyzing carotenoids in bird tissues
Kevin McGraw and Matthew Toomey
15. Analysis of plant-derived carotenoids in camouflaging stick insects
Royce Cumming, Kaijie Zhu, Eleanore T. Wurtzel and Jeffry Petracca

About the author

As a Ph.D student, Eleanore Wurtzel innovated gene tagging and isolated the first genes for two-component signaling in bacteria, laying the foundation for study of signaling mechanisms found throughout nature, including plants. With an NSF postdoctoral fellowship, Dr. Wurtzel boldly changed fields from bacterial membrane biochemistry to plant biology, when maize was the only model system. She established some of the first experiments on plant chromatin structure as an NSF Plant Biology postdoctoral fellow at Brookhaven National Laboratory. She then joined Cold Spring Harbor Laboratory and began research on maize carotenoid biosynthesis, then a poorly studied area. Dr. Wurtzel next joined the Biological Sciences Department at Lehman College, City University of New York, where she is currently a Full Professor and on the faculty of the CUNY Biology and Biochemistry PhD programs. Eleanore Wurtzel has made fundamental and longstanding contributions to the field of plant carotenoid biosynthesis, plant biochemistry, and plant metabolic engineering which are enabling improvement of crops for sustainable solutions to global vitamin A deficiency affecting the health and mortality of 250 million children worldwide. Dr. Wurtzel is grateful to the many students, postdocs, and visiting scientists who have contributed to her laboratory’s research for which she has been recognized as a Fellow of AAAS, Fellow of ASPB, and most recently as a Fellow of the International Carotenoid Society. Dr. Wurtzel serves as a Monitoring Editor of Plant Physiology. Dr. Wurtzel has also been a long-standing elected member of the Gordon Research Conferences (GRC) Board of Trustees. She has been instrumental at GRC in developing and contributing to programs for women in science. She also founded and chaired the first GRC on Plant Metabolic Engineering and founded the GRC seminar for early career scientists for both the GRC Plant Metabolic Engineering community and the GRC Carotenoids community.

Product details

• Assisted by: Eleanore Wurtzel (Editor), Eleanore T Wurtzel (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Released: 31.07.2022
• EAN: 9780323999755
• ISBN: 978-0-323-99975-5
• No. of pages: 544
• Series: Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; SCIENCE / Life Sciences / Biochemistry, SCIENCE / Life Sciences / Molecular Biology, biochemistry, molecular biology