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This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E. coli (STEC) and Shiga toxins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Shiga Toxin-Producing E. coli: Methods and Protocols aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis.
List of contents
Integrated Approach for the Diagnosis of Shiga Toxin-producing Escherichia coli Infections in Humans.- Shiga Toxin-producing E. coli in Animals - Detection, Characterisation, and Virulence Assessment.- Identification of Shiga Toxin-producing Escherichia coli Outbreaks using Whole Genome Sequencing.- Predicting Host Association for Shiga Toxin-producing E. coli serogroups by Machine Learning.- Isolation and Characterization of Shiga Toxin Bacteriophages.- Lambda Red Mediated Recombination in Shiga toxin-producing Escherichia coli.- Functional Analysis of Shiga Toxin-producing Escherichia coli Biofilm Components in Plant Leaves.- Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-producing Escherichia coli Outer Membrane Vesicles.- Isolation and Characterization of Shiga Toxin-Associated Microvesicles.- Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Receptors.- Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-producing Escherichia coli to Epithelial Cells.- Determining Shiga Toxin-producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber.- Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions.- Use of the Dynamic TIM-1 Model for an In-depth understanding of the Survival and Virulence Gene Expression of Shiga toxin-producing Escherichia coli in the Human Stomach and Small Intestine.- Measuring Effector-mediated Modulation of Inflammatory Responses to Infection with Enteropathogenic and Shiga toxin-producing E. coli.- Interaction of Bovine Lymphocytes with Products of Shiga toxin-Producing Escherichia coli.- Infection of Immunocompetent Conventional Mice with Shiga toxin-producing E. coli: The DSS+STEC Model.- Infant Rabbit Model for Studying Shiga Toxin-producing Escherichia coli.- Citrobacter rodentium lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-producing E. coli Infection.- Overview of the Effect of Citrobacter rodentium Infection On Host Metabolism And The Microbiota.
