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This book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Photomorphogenesis: Methods and Protocols serves as an ideal guide for researchers and students who are new to the field, as well as a stepping stone for experienced researchers to further their skills in this fast-developing field.
List of contents
Setting up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity.- Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis.- Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis.- Isolation of UVR8 Protein Complexes.- Phenotypic Study of Photo-Morphogenesis in Arabidopsis Seedlings.- Experimental Procedures for Studying Skotomorphogenesis in Arabidopsis thaliana.- Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq).- Co-Immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners.- Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato.- Characterization of Seedling Greening Process in Plant Photomorphogenesis.- Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody.- Uncover the Nuclear Proteomic Landscape with Enriched Nuclei Followed by Label-Free Quantitative Mass Spectrometry.- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.- Co-Immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants.- Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay.- Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System.- Semi-In-Vivo Pull Down Assay for Blue Light-Dependent Protein Interactions.- Tobacco System for Studying Protein Colocalization and Interactions.
