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This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.
List of contents
Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells.- Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach.- Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes.- Functional Characterization of SLC transporters using Solid-Supported-Membranes.- Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents.- Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance.- Examining Membrane Proteins by Neutron Scattering.- Solution X-ray Scattering for Membrane Protein.- Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide.- Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers.- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.- Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS).- Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scale.- Short-Range Distances Measurement by Transition Metal Ion FRET.- PELDOR/DEER - An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers.
