PCR/RT- PCR in situ - Light and Electron Microscopy

List of contents

General Introduction Chapter 1 - General Principles Chapter 2 - Preparation of Samples Chapter 3 - Pretreatments Chapter 4 - Reverse Transcription (RT) Chapter 5 - Polymerase Chain Reation (PCR) Chapter 6 - Hybridization Chapter 7 - Revelation Chapter 8 - Electron Microscopy Chapter 9 - Controls and Problems Chapter 10 - Typical Protocols Chapter 11 - Examples of Observations

About the author

Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.

Summary

Covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two fresh approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. This title provides theoretical consideration, as well as practical approaches to in situ PCR.

Product details

• Authors: Gerard Morel, Morel Gerard, Mireille Raccurt, Raccurt Mireille
• Assisted by: Gerard Morel (Editor of the series)
• Publisher: Taylor and Francis
• Languages: English
• Product format: Hardback
• Released: 27.09.2002
• EAN: 9780849300417
• ISBN: 978-0-8493-0041-7
• No. of pages: 444
• Weight: 1014 g
• Illustrations: schwarz-weiss Illustrationen, farbige Illustrationen, Raster,schwarz-weiss
• Series: Methods in Visualization
• Subjects: Natural sciences, medicine, IT, technology > Natural sciences (general); Microscopy, SCIENCE / Life Sciences / Genetics & Genomics, SCIENCE / Microscopes & Microscopy, Genetics (non-medical)