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This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans.- Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification.- CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia.- Analysis of Epithelial Cell Responses to Microbial Pathogens.- Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry.- Fungal Bioreporters to Monitor Outcomes of Blastomyces - Host Cell Interactions.- Fungal Bioreporters to Monitor Outcomes of Aspergillus - Host Cell Interactions.- Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays.- Experimental Evolution of Candida by Serial Passaging in Host Cells.- Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells.- Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras.- Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole.- Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections.- A Murine Model for Chronic A. fumigatus Airway Infections.- Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge.
