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This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
List of contents
Methods of Development of Biparental Mapping Populations in Horticultural Crops.- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits.- Using Metabolomics to Assist Plant Breeding.- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications.- High Resolution Melting Analysis as a Tool for Plant Species Authentication.- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods.- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome.- Association Mapping in Plants.- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs).- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis.- RNA Interference (RNAi) in Tomato Crop Reseserch.- Protoplast Based Method for Genome Editing in Tetraploid Potato.- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria.- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant.- Assembly of TALEN and mTALE-Act for Plant Genome Engineering.- Genome Editing to Achieve the Crop Ideotype in Tomato.- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging.- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.
